lexsy neo antibiotic (Jena Bioscience)
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Jena Bioscience
lexsy neo antibiotic
Lexsy Neo Antibiotic, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lexsy+neo+antibiotic/LEXSY+Neo/pmc05251173-117-7-20
Average 94 stars, based on 21 article reviews
Lexsy Neo Antibiotic, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lexsy+neo+antibiotic/LEXSY+Neo/pmc05251173-117-7-20
Average 94 stars, based on 21 article reviews
lexsy neo antibiotic - by Bioz Stars,
2026-09
94/100 stars
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Clone Assay:Article Title: The role of GlcNAc-PI-de-N-acetylase gene by gene knockout through homologous recombination and its consequences on survival, growth and infectivity of Leishmania major in in vitro and in vivo conditions. Article Snippet: It was pulsed with Multiporator (Eppendorf, Germany) for d=2 mm cuvettes at 1000 V and 160 μsec and returned to wet ice for exactly 10 min. Electroporated parasites were kept in a liquid medium without antibiotics for one day prior to selection. .. To isolate the clones that contained the resistance gene, transfected parasites were gathered by centrifugation (3000 rpm, 10 min, at 4 0C) and plated on to semi-solid DMEM media containing 1% agar in the presence of 50μg/ml Article Title: Designing and Cloning Molecular Constructs to Knock Out N-Acetylglucosamine Phosphatidylinositol De-N-Acetylas e (GPI12) Gene in Leishmania major (MRHO/IR/75/ER) Article Snippet: .. To identify the resistant clones, transfected parasites were centrifuged (3000 rpm, 10 min, 4 °C) and plated onto semi-solid DMEM media which contained 1% noble agar in the presence of 50 μg/ml Article Title: The role of GlcNAc-PI-de-N-acetylase gene by gene knockout through homologous recombination and its consequences on survival, growth and infectivity of Leishmania major in in vitro and in vivo conditions. Article Snippet: .. For the second round of the transfection, both Transfection:Article Title: The role of GlcNAc-PI-de-N-acetylase gene by gene knockout through homologous recombination and its consequences on survival, growth and infectivity of Leishmania major in in vitro and in vivo conditions. Article Snippet: It was pulsed with Multiporator (Eppendorf, Germany) for d=2 mm cuvettes at 1000 V and 160 μsec and returned to wet ice for exactly 10 min. Electroporated parasites were kept in a liquid medium without antibiotics for one day prior to selection. .. To isolate the clones that contained the resistance gene, transfected parasites were gathered by centrifugation (3000 rpm, 10 min, at 4 0C) and plated on to semi-solid DMEM media containing 1% agar in the presence of 50μg/ml Article Title: Designing and Cloning Molecular Constructs to Knock Out N-Acetylglucosamine Phosphatidylinositol De-N-Acetylas e (GPI12) Gene in Leishmania major (MRHO/IR/75/ER) Article Snippet: .. After the second round of transfection, both Article Title: Designing and Cloning Molecular Constructs to Knock Out N-Acetylglucosamine Phosphatidylinositol De-N-Acetylas e (GPI12) Gene in Leishmania major (MRHO/IR/75/ER) Article Snippet: .. To identify the resistant clones, transfected parasites were centrifuged (3000 rpm, 10 min, 4 °C) and plated onto semi-solid DMEM media which contained 1% noble agar in the presence of 50 μg/ml Article Title: The role of GlcNAc-PI-de-N-acetylase gene by gene knockout through homologous recombination and its consequences on survival, growth and infectivity of Leishmania major in in vitro and in vivo conditions. Article Snippet: .. For the second round of the transfection, both Centrifugation:Article Title: The role of GlcNAc-PI-de-N-acetylase gene by gene knockout through homologous recombination and its consequences on survival, growth and infectivity of Leishmania major in in vitro and in vivo conditions. Article Snippet: It was pulsed with Multiporator (Eppendorf, Germany) for d=2 mm cuvettes at 1000 V and 160 μsec and returned to wet ice for exactly 10 min. Electroporated parasites were kept in a liquid medium without antibiotics for one day prior to selection. .. To isolate the clones that contained the resistance gene, transfected parasites were gathered by centrifugation (3000 rpm, 10 min, at 4 0C) and plated on to semi-solid DMEM media containing 1% agar in the presence of 50μg/ml Selection:Article Title: Designing and Cloning Molecular Constructs to Knock Out N-Acetylglucosamine Phosphatidylinositol De-N-Acetylas e (GPI12) Gene in Leishmania major (MRHO/IR/75/ER) Article Snippet: .. After the second round of transfection, both Article Title: The role of GlcNAc-PI-de-N-acetylase gene by gene knockout through homologous recombination and its consequences on survival, growth and infectivity of Leishmania major in in vitro and in vivo conditions. Article Snippet: .. For the second round of the transfection, both |